H&I: BIOCHEMISTRY

ESSENTIAL READING 

1. HAEM METABOLISM
Chapter 21 of Lippincott’s Illustrated Reviews:Biochemistry Sixth Edition
Conversion of Amino Acids to Specialized Products

2. METABOLISM IN ERYTHROCYTES
Chapter 3 of of Lippincott’s Illustrated Reviews:Biochemistry Sixth Edition
Globular Proteins; Read Methemoglobinemia, role of 2,3 BPG in oxygen transport.

 Chapter 13 of of Lippincott’s Illustrated Reviews:Biochemistry Sixth Edition
Pentose Phosphate Pathway and Nicotinamide Adenine Dinucleotide Phosphate

 3. Self Directed Learning:

1. Explain how Lead affects haem synthesis.
2. State the enzyme defects and briefly describe the classification of porphyrias.
3. Explain the basis of photosensitivity and neurological symptoms of porphyrias.
4. List investigations done to diagnose porphyria.

 4. Self Directed Learning:

1. Explain the biochemical basis of the hemolytic anemia observed in deficiency of erythrocyte pyruvate kinase .
2. Outline the Rapaport Leubering Shunt and explain the role of 2,3 BPG.
3. List the causes of methemoglobinemia.
4. Discuss the reactions catalysed by Diaphorase 1 and Diaphorase 2.
5. Outline the Pentose Phosphate pathway and explain the role of this pathway in erythrocytes

5. Self Directed Learning:

1. Explain difference between serum and plasma.
2. List the proteins that are present in plasma but are absent in serum. Explain why they are not present in serum.
3. What is the clinical utility of serum protein electrophoresis.